Tissue Bank FAQ

English Springer Spaniel Cancer Tissue Bank – FAQs

 

  1. The Tissue Collection Protocol requires that a kit be sent from the University of Missouri to the attending veterinarian. What is in the kit and is there a charge?

Answer: The kit will include the paperwork and instructions for collection, formalin containers or cryovials for tissue to be flash frozen, cassettes, box, shipping labels and other items the veterinarian will need to send the tissue to the lab. It will not include the dry ice needed to ship tissue that was flash frozen. If a tissue sample is smaller than the recommended size, the kit can include biopsy paper at the veterinarian’s request. Tissue that can be flash frozen is preferred, but it is recognized that this may not be possible. If the lab does not have a 48-hour advanced notice to send the kit, these items would not be provided. (Note: If this situation exists, the vet will need to put the specimen in a tube to freeze immediately, or put the specimen in formalin. If this isn’t done, the specimen will not be of sufficient quality.)

There is no charge for the kit for the 1st 100 samples collected. That cost will be funded by the English Springer Spaniel Field Trial Association Foundation unless the client chooses to pay that amount.

  1. How is the dry ice to be provided?

Answer: Dry ice will be acquired by the owner and is available from multiple sources such as grocery stores or ice companies. Dry ice is only required if tissue is flash frozen at temperatures between -80 to -150 degrees. If the tissue is formalin fixed, no dry ice is required.

  1. What procedure should be followed if there is not a 48-hour period to request and receive the kit?

Answer: The tissue can still be collected. The vet should contact the University of Missouri’s OneHealth Biorepository Supervisor, Jennifer Schnabel at (573) 884-2093 and/or mubiobank@missouri.edu for instructions and shipping information. Once a specimen has been removed, it must be flash frozen within 30 minutes OR put into formalin within an hour. Flash frozen tissue must be shipped on dry ice. If it is formalin fixed, the container can be shipped to the Biorepository.

  1. Can tissue be collected from a dog that has been euthanized?

Answer: Yes, it can. Please refer to the directions in #3.

  1. If at the time of surgery to remove a mass, it is not known whether the tissue is, in fact, malignant, should tissue still be sent to the lab?

Answer: Yes, it should be, following all the general procedures in the protocol. When a pathology report is received after the fact, it should be forwarded to the University regardless of whether the mass is malignant or benign. The University may still find the tissue useful in comparing other benign or malignant tumors of a similar type.

  1. What if the sample size is smaller than the amount specified in the protocol?

Answer: If the sample is small, the kit can include biopsy paper to assist in the preservation.

  1. Will smaller practices be able to handle these requirements?

Answer: Yes! Practices without access to a -80 or -150 freezer can preserve the tissue in formalin that can be shipped at room temperature. The Foundation and the University are identifying larger practices, oncology specialists, teaching universities and other veterinarians that are candidates for having kits shipped in advance to have on hand.

 

The goal of the Tissue Bank is to obtain specimens of any type of cancer. Use of this tissue in research projects will allow for the determination of biomarkers to identify cancers early and to develop drugs and other cures. The Foundation will be working with the University as tissue is submitted to determine what additional information can be disseminated to assist owners and veterinarians with the collection and shipment procedures.